Irwan Hanish

Back to Serology and Other Immunologic Techniques

Practice Questions for Serology

Answer each question from memory first, aloud or on paper. Then rewatch Serology and Other Immunologic Techniques to check yourself.

  1. Explain the significance of serology and how it contributes to disease diagnosis.
  2. Compare and contrast radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) in terms of their methodology and practical applications.
  3. Describe the principle and steps involved in conducting a sandwich ELISA, and explain why it is referred to as a ‘sandwich’.
  4. Discuss the role of the primary and secondary antibodies in the ELISA technique and the importance of the washing steps.
  5. Outline the process of immunoprecipitation and how it enables the isolation of a specific antigen from a mixture.
  6. Explain immuno-affinity chromatography and how it differs from immunoprecipitation in terms of technique and result.
  7. Describe the four main steps of Western blotting and the purpose of each step in the detection of proteins.
  8. Discuss the use of flow cytometry in immunology, including how it differentiates and sorts cells.
  9. Explain the principle of hemagglutination and its application in determining ABO blood groups for transfusions.
  10. Discuss the immunological considerations of the Rhesus factor in pregnancy and the purpose of administering RhoGAM injections.